26.6 B12

26.6.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 113.7952111 1 <0.0001 ***
B12 0.0039381 1 0.9 ns
Study 0.1835478 1 0.7 ns
GA_samp 132.0512503 1 <0.0001 ***
B12:Study 0.4161577 1 0.5 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 24.428879 1 <0.0001 ***
B12 1.133261 1 0.3 ns
Study 3.998083 1 0.05 ns
GA_samp 1648.072726 1 <0.0001 ***
B12:Study 0.017806 1 0.9 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 336.340208 1 <0.0001 ***
B12 6.624419 1 0.01 *
Study 1.872150 1 0.2 ns
GA_samp 815.254269 1 <0.0001 ***
B12:Study 1.840691 1 0.2 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 4.297 4.297 0.04 *
3 Study 1 Inf 7.878 7.878 0.005 **
4 GA_samp 1 Inf 0.122 0.122 0.7 ns
2 B12:Study 1 Inf 1.131 1.131 0.3 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 3.806 3.806 0.05 ns
3 Study 1 Inf 11.788 11.788 0.0006 ***
4 GA_samp 1 Inf 0.209 0.209 0.6 ns
2 B12:Study 1 Inf 1.079 1.079 0.3 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 B12 1 Inf 2.841 2.841 0.09 ns
3 Study 1 Inf 30.734 30.734 <0.0001 ***
4 GA_samp 1 Inf 0.333 0.333 0.6 ns
2 B12:Study 1 Inf 0.595 0.595 0.4 ns

26.6.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 12.05 (7.69, 18.87) <0.0001
B12 1.00 (1.00, 1.00) 0.41
StudySTOP 1.01 (0.88, 1.16) 0.87
GA_samp 1.17 (1.14, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.81 (1.44, 2.28) <0.0001
B12 1.00 (1.00, 1.00) 0.04
StudySTOP 1.14 (1.06, 1.22) 0.0003
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.04, 0.08) <0.0001
B12 1.00 (1.00, 1.00) 0.01
StudySTOP 1.02 (0.93, 1.12) 0.66
GA_samp 1.32 (1.29, 1.34) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 18.19 (9.98, 33.15) <0.0001
B12 1.00 (1.00, 1.00) 0.04
StudySTOP 0.72 (0.60, 0.86) 0.0003
GA_samp 0.99 (0.96, 1.03) 0.71
HOMA-IR
Var Effect P
(Intercept) 4.54 (2.43, 8.49) <0.0001
B12 1.00 (1.00, 1.00) 0.06
StudySTOP 0.62 (0.51, 0.75) <0.0001
GA_samp 0.99 (0.95, 1.03) 0.63
Glucose (mmol/L)
Var Effect P
(Intercept) 5.67 (5.09, 6.31) <0.0001
B12 1.00 (1.00, 1.00) 0.10
StudySTOP 0.87 (0.84, 0.90) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.57

26.6.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 96.44883 7.991495 103.34357 81.83386 113.6739
STOP 97.57641 7.546418 78.86473 83.65432 113.8155
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 90.37671 2.675871 190.0294 85.24966 95.81211
STOP 102.98997 2.681540 114.0150 97.81254 108.44145
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.305636 0.0775524 424.6029 2.158132 2.463223
STOP 2.354790 0.0636642 207.0318 2.232563 2.483709
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.15732 0.8882227 Inf 12.519214 16.00976
STOP 10.17033 0.4597934 Inf 9.307926 11.11264
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.420104 0.2279209 Inf 3.001332 3.897308
STOP 2.123460 0.1003240 Inf 1.935658 2.329483
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.406117 0.0673630 Inf 5.275688 5.539772
STOP 4.711955 0.0397875 Inf 4.634615 4.790586

26.6.4 Comparisons

Effect for every 100 unit increase in B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) B12363.5 / B12263.5 SCOPE 1.01 (0.98, 1.05) 0.4
PRL (ng/ml) B12363.5 / B12263.5 STOP 1.01 (0.98, 1.05) 0.4
hPL (μg/ml) B12363.5 / B12263.5 SCOPE 1.02 (1.00, 1.04) 0.04
hPL (μg/ml) B12363.5 / B12263.5 STOP 1.02 (1.00, 1.04) 0.04
GH2 (ng/ml) B12363.5 / B12263.5 SCOPE 1.03 (1.01, 1.05) 0.01
GH2 (ng/ml) B12363.5 / B12263.5 STOP 1.03 (1.01, 1.05) 0.01
Insulin (uU/ml) B12363.5 / B12263.5 SCOPE 0.95 (0.91, 1.00) 0.04
Insulin (uU/ml) B12363.5 / B12263.5 STOP 0.95 (0.91, 1.00) 0.04
HOMA-IR B12363.5 / B12263.5 SCOPE 0.95 (0.90, 1.00) 0.06
HOMA-IR B12363.5 / B12263.5 STOP 0.95 (0.90, 1.00) 0.06
Glucose (mmol/L) B12363.5 / B12263.5 SCOPE 0.99 (0.99, 1.00) 0.10
Glucose (mmol/L) B12363.5 / B12263.5 STOP 0.99 (0.99, 1.00) 0.10

26.6.5 Estimated means (50 unit increase)

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 96.12350 7.943488 102.26730 81.59159 113.2436
STOP 97.24727 7.514380 78.60051 83.38271 113.4172
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 89.98138 2.648226 185.8123 84.90570 95.36048
STOP 102.53947 2.664550 113.1569 97.39417 107.95658
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.288290 0.0761590 410.6529 2.143372 2.443006
STOP 2.337074 0.0630674 206.3572 2.215985 2.464780
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.33738 0.8943328 Inf 12.687433 16.20189
STOP 10.29968 0.4592552 Inf 9.437771 11.24031
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.463888 0.2293611 Inf 3.042297 3.943901
STOP 2.150644 0.1002700 Inf 1.962830 2.356429
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.415384 0.0667619 Inf 5.286101 5.547829
STOP 4.720032 0.0401342 Inf 4.642022 4.799353

26.6.6 Comparisons (50 unit increase)

Effect for every 50 unit increase in B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) B12313.5 / B12263.5 SCOPE 1.01 (0.99, 1.02) 0.4
PRL (ng/ml) B12313.5 / B12263.5 STOP 1.01 (0.99, 1.02) 0.4
hPL (μg/ml) B12313.5 / B12263.5 SCOPE 1.01 (1.00, 1.02) 0.04
hPL (μg/ml) B12313.5 / B12263.5 STOP 1.01 (1.00, 1.02) 0.04
GH2 (ng/ml) B12313.5 / B12263.5 SCOPE 1.02 (1.00, 1.03) 0.01
GH2 (ng/ml) B12313.5 / B12263.5 STOP 1.02 (1.00, 1.03) 0.01
Insulin (uU/ml) B12313.5 / B12263.5 SCOPE 0.98 (0.95, 1.00) 0.04
Insulin (uU/ml) B12313.5 / B12263.5 STOP 0.98 (0.95, 1.00) 0.04
HOMA-IR B12313.5 / B12263.5 SCOPE 0.97 (0.95, 1.00) 0.06
HOMA-IR B12313.5 / B12263.5 STOP 0.97 (0.95, 1.00) 0.06
Glucose (mmol/L) B12313.5 / B12263.5 SCOPE 1.00 (0.99, 1.00) 0.10
Glucose (mmol/L) B12313.5 / B12263.5 STOP 1.00 (0.99, 1.00) 0.10

26.6.7 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.6.8 Plots